Design of small molecule reagents that enable signal amplification via an autocatalytic, base-mediated cascade elimination reaction.
نویسندگان
چکیده
This Communication describes three small molecule reagents that amplify the signal for a detection event via an autocatalytic reaction. Two signals are obtained from each reagent: (i) the dibenzofulvene chromophore and (ii) piperidine, which can be visualized using a pH indicator dye. The reagents are demonstrated in a model assay for palladium.
منابع مشابه
A Strategy for Minimizing Background Signal in Autoinductive Signal Amplification Reactions for Point-of-Need Assays.
Rapid point-of-need assays are used to detect abundant biomarkers. The development of in situ signal amplification reactions could extend these assays to screening and triaging of patients for trace levels of biomarkers, even in resource-limited settings. We, and others, have developed small molecule-based in situ signal amplification reactions that eventually may be useful in this context. Her...
متن کاملA small molecule sensor for fluoride based on an autoinductive, colorimetric signal amplification reaction.
This article describes a small molecule reagent that is capable of detecting fluoride down to 0.12 mM (2.3 ppm) in water. The reagent reveals this level of fluoride through a novel autoinductive signal amplification reaction that produces an unambiguous colorimetric readout.
متن کاملA target-activated autocatalytic DNAzyme amplification strategy for the assay of base excision repair enzyme activity.
Based on a target-activated autocatalytic DNAzyme amplification strategy, novel fluorescence sensing platforms were constructed for highly sensitive and selective assay of base excision repair enzyme activity. By using a rolling circle amplification (RCA)-coupled amplification cascade, an extremely low detection limit (0.002 U mL(-1)) was achieved.
متن کاملDirecting otherwise incompatible reactions in a single solution by using DNA-templated organic synthesis.
General methods for translating amplifiable information carriers such as DNA into synthetic molecules may enable the evolution of non-natural molecules through iterated cycles of translation, selection, and amplification that are currently available only to proteins and nucleic acids. During the process of developing such a method, we recently discovered that DNA templates can sequence-specific...
متن کاملCatalysts of DNA Strand Cleavage at Apurinic/Apyrimidinic Sites
Apurinic/apyrimidinic (AP) sites are constantly formed in cellular DNA due to instability of the glycosidic bond, particularly at purines and various oxidized, alkylated, or otherwise damaged nucleobases. AP sites are also generated by DNA glycosylases that initiate DNA base excision repair. These lesions represent a significant block to DNA replication and are extremely mutagenic. Some DNA gly...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 48 24 شماره
صفحات -
تاریخ انتشار 2012